Biochemical analysis of transcriptional repression bydrosophila histone deacetylase 1*

HIGHLIGHTS

• who: Xuejun Huang from the Transcription Factors and DNA Templates-His6-tagged NF-␬B p, protein (29) was synthesized in Sf, cells by using a recombinant baculovirus kindly provided by DrJ. Hiscott (Lady Davis Institute, Montreal, Canada). The p, protein was synthesized and purified essentially as described for His6-tagged p, (30, ). FLAG-tagged Sp, was purified by using a recombinant baculovirus that was kindly provided by Drs. Soojin Ryu and Robert Tjian (University of California, Berkeley, CA). Constructions that encode His6-tagged versions of full-length d, and Gal4-d, were prepared from the d, cDNA . . .
  

   

  

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