Opening of the outer membrane protein channel in tripartite efflux pumps is induced by interaction with the membrane fusion partner*

HIGHLIGHTS

• who: Thamarai K. Janganan from the (UNIVERSITY) have published the research: Opening of the Outer Membrane Protein Channel in Tripartite Efflux Pumps Is Induced by Interaction with the Membrane Fusion Partner*, in the Journal: (JOURNAL) of February/18,/2011

SUMMARY

Modeling studies, supported by cross-link- Site-directed Mutagenesis of mtrE-Site-directed mutagenesis was performed with the QuickChange XL kit (Stratagene). Plasmids were extracted from the colonies and their DNA sequenced. The E. coli cells harboring plasmids were grown at 37 °C in 2xYT media, with shaking at 220 rpm. Cell paste . . .

 



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