P53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (er) stress*

HIGHLIGHTS

• who: Sally E. Thomas from the EXPERIMENTAL PROCEDURES Expression Plasmids-The coding sequence of p53/p, was subcloned from pcDNA31.p53/p47⌬IRES (a gift from Prof. Robin Fahraeus, INSERM, France) into pEGFP-, (Clontech) between HindIII and SalI restriction sites. The coding sequence of full-length p, was subcloned from pcDNA3.1.p53wt (Prof. Robin Fahraeus, INSERM, France) into pEGFP-, between BglII and SalI sites. Cell Culture-, p53⫹/⫹ and p53⫺/⫺ cells stably transfected with inducible , Tet-On small interfering hairpin RNA (shRNAi) or scrambled control shRNAi were a kind gift from Dr. Giovanna Damia (Milan, Italy) (27 . . .
  

   

  

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