Early markers of sickle nephropathy in children with sickle cell anemia are associated with red cell cation transport activity

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• who: John Brewin et al. from the Laboratory assays RBC samples were washed in simple, (N-morpholino) propanesulfonic acid (MOPS)-buffered saline, comprising (in mM): , NaCl, KCl, CaCl2, MOPS, glucose, pH, ., at, °C. Oxygen tension was controlled using a Wösthoff gas mixing pump with RBCs incubated in Eschweiler tonometers. RBC permeability was assessed using radioactive tracers ( Rb+) to measure activity of the main cation transport systems involved in RBC dehydration: KCC, Gardos channel and Psickle. KCC was measured as Cl, dependent K+ transport using , to substitute for Cl . The Gardos channel was measured as clotrimazole-sensitive . . .
  

   

  

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