Common alterations in pbp1a from resistant streptococcus pneumoniae decrease its reactivity toward β-lactams

HIGHLIGHTS

• who: Viviana Job from the EXPERIMENTAL PROCEDURES Plasmids and Site-directed Mutagenesis-Pneumococcal strains, , (University Hospital, Grenoble, France), and , were used as sources of genomic DNA for the amplification of pbp1a genesThe fragments encoding the extracellular region of PBP1a (residues, henceforth noted PBP1a) were PCR-amplified and introduced as BamHI/ XhoI fragments in place of the pbp2x fragment in pGEX-Spbp2x*-f, (21). This vector had been previously modified by site-directed mutagenesis to eliminate a second undesired BamHI site (GGATCC into GGGTCC) in the f, region. Site-directed mutagenesis of pbp1a genes from strains , and, to . . .
  

   

  

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