Regulation of urokinase/urokinase receptor interaction by heparin-like glycosaminoglycans*

HIGHLIGHTS

• who: Marco Pucci from the Cell Culture, Cell Transfections with the Human u-PAR Construct, and Incorporation of Radioactive Precursors-Human skin fibroblasts (HSF) from skin biopsies and Chinese hamster ovary fibroblasts (CHO, ) were grown as monolayers at , °C, in, cm, dishes in Dulbecco's modified Eagle's medium supplemented with, % fetal calf serum, mM, glutamine, and penicillin/streptomycinThe pgsA , clone of CHO fibroblasts, which lacks the xylosyltransferase that initiates GAGs synthesis, was kindly provided by Dr. J. D. Esko, of the University of Alabama at Birmingham. Both parental CHO and the pgsA clone were stably cotransfected . . .
  

   

  

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