Characterization of gdp-mannose dehydrogenase from the brown alga ectocarpus siliculosus providing the precursor for the alginate polymer

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• who: Raimund Tenhaken from the Enzyme Assay-The routine enzyme assay is based on the increase of NADH measured as an increase in , nmThis can be performed easily in microcuvettes as well as in microtiter plates. The standard assay buffer consisted of , mM Tris-glycine buffer, pH, .75, mM NAD⫹, ., mM GDP-Man, to which the Es, enzyme (typically , ␮g) was added. We compared the kinetic parameters of the MBP-Es, fusion protein with the TEV-cleaved Es, alone but did not find any differences in the catalytic properties. Therefore, the MBP fusion protein was used routinely . . .
  

   

  

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