Maximising the use of scarce qpcr master mixes

HIGHLIGHTS

  • who: Stephen Bustin et al. from the Medical Technology Research Centre, Anglia Ruskin University, Chelmsford , SQ, UK have published the article: Maximising the Use of Scarce qPCR Master Mixes, in the Journal: (JOURNAL)
  • what: In this communication the authors propose two universal and unanticipated adaptations that are useful not just for diagnostic testing but can be implemented for any qPCR-based experiment.
  • how: Similar amplification results were obtained with all four master mixes when target DNA concentrations were increased 50-fold . These results indicated that reducing the concentration of the master mix did . . .

     

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