P450bm3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations

HIGHLIGHTS

  • who: Nina Beyer from the Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh, AG Groningen, The Netherlands have published the research work: P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations, in the Journal: (JOURNAL)
  • what: The authors report a higher turnover for the wild-type P450BM3 without any cofactor recycling system (16%) and a slightly higher turnover for the fusion enzyme (25-26%). The rate of NAD+ reduction by PTDH was lower than the rate of NADH oxidation by the P450, as the authors report for the PTDH . . .

     

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