Requirement for reactive oxygen species in serum-induced and platelet-derived growth factor-induced growth of airway smooth muscle*

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  • who: Sukhdev S. Brar from the Materials-Male adult Sprague-Dawley rats were purchased from Charles River (Raleigh, NC)Transgenic mice lacking the gene for the gp, component of phagocyte oxidase (gp91phox) were a gift from Dr. Mary Dinauer (Indiana University, Indianapolis, IN) (16). Protease inhibitors and guanidine thiocyanate were obtained from Roche Molecular Biochemicals. Diphenyleneiodonium chloride was from Aldrich. Dulbecco's modified Eagle's medium (DMEM), Hanks' balanced salt solution (HBSS), HEPES, antibiotic-antimycotic (10, units of penicillin, , mg of streptomycin, and , mg of amphotericin B/ml) and , The abbreviations used are: DMEM, Dulbecco's modified . . .

     

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