The brucella abortus general stress response system regulates chronic mammalian infection and is controlled by phosphorylation and proteolysis*

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  • who: Hye-Sook Kim from the EXPERIMENTAL PROCEDURES Bacterial Strains and Culture Conditions-All studies in live Babortus were performed at Biosafety Level , (BSL3) as per CDC select agent regulations at the University of Chicago Howard Taylor Ricketts Laboratory or East Carolina University School of Medicine. A detailed description of bacterial strains and culture conditions is provided in the supplemental Materials and Methods. Construction of B. abortus Mutants and Genetic Complementation-All primers used in this study are listed in Table, . A double recombination strategy described previously (20, ) was used to create unmarked B. abortus , mutant strains . . .

     

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