Mechanism of tyrosine phosphorylation and activation of phospholipase c-ù³1

HIGHLIGHTS

• who: Fujio Sekiya from the Materials and Cell Lines-PDGF-BB (human recombinant) and purified active tyrosine kinases (EGFR, ZAP-70, and Src) were obtained from Upstate BiotechnologyEGF (human recombinant) and , were from Calbiochem. Null-, cells, which are derived from PLC␥1⫺/⫺ mouse embryonic fibroblasts (17), were kindly provided by G. Carpenter (Vanderbilt University School of Medicine, Nashville, TN). NIH , /EGFR cells were as described previously (18). Null-TV1, and NIH , /EGFR cells were maintained under a humidified atmosphere of, % , in Dulbecco's modified Eagle's medium supplemented with penicillin-streptomycin and either, % fetal bovine serum (Null . . .
  

   

  

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