The adaptor protein hsh2 attenuates apoptosis in response to ligation of the b cell antigen receptor complex on the b lymphoma cell line, wehi-231*

HIGHLIGHTS

• who: Brantley R. Herrin from the Cells and Cell Culture-, murine B lymphoma cells and Phoenix gp retroviral packaging cells (provided by Gary Nolan, Department of Microbiology and Immunology, Stanford University, Palo Alto, CA) were cultured in RPMI , medium supplemented with, % fetal bovine serum (HyClone, Logan, UT), ␮M, glutamine, ␮M, mercapto-ethanol, ␮g/ml streptomycin-penicillin, and , ␮g/ml gentamicin (Sigma) at , °C under, % CO Antibodies-, cells were stimulated with polyclonal goat anti-mouse IgM (F(ab⬘)2) Ab purchased from BIOSOURCE International (Camarillo, CA). For Western blot analysis, the following antibodies were purchased: mouse anti-FLAG . . .
  

   

  

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