Protein kinase a (pka) phosphorylation of shp2 protein inhibits its phosphatase activity and modulates ligand specificity

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• who: Brian T. Burmeister from the Anti-phospho-PKA substrate (RRXS*/T*) antibody (rabbit, :1000) was from Cell Signaling TechnologyAnti-phosphotyrosine antibody, clone , (mouse, :1000), and anti-GST (mouse, :1000) were from EMD Millipore. Wild-type Shp, mammalian expression constructs were provided by Dr. GenSheng Feng (University of California, San Diego). Mammalian expression constructs for GAB1, GAB2, and constitutively active Src (Y F) were provided by Dr. Andrei Karginov (University of Illinois at Chicago). Bacterial Expression-GST and GST-Shp2-, domain proteins were expressed as, terminal GST-tagged fusions using the pGEX- , vector (Amersham Biosciences) in bacteria . . .
  

   

  

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