A versatile, efficient strategy for assembly of multi-fragment expression vectors in saccharomyces cerevisiae using 60 bp synthetic recombination sequences

HIGHLIGHTS

  • who: TECHNICAL NOTES et al. from the Department of Biotechnology, Delft University of Technology, Julianalaan, Delft , BC, The Netherlands have published the paper: A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences, in the Journal: (JOURNAL)
  • what: To meet the above requirements, the aim of this study was to reduce the incidence of incorrect plasmid assembly and to make a robust, versatile in_vivo assembly strategy for multi-component plasmids. While implementing in_vivo assembly in the research , the authors have been surprised by its simplicity . . .

     

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