HIGHLIGHTS
- who: Salete M. Newton from the Salete MNewton, Vy Trinh, Hualiang Pi, and Phillip E. Klebba, From the Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma , When Gram-negative bacteria acquire iron, the metal crosses both the outer membrane (OM) and the inner membrane, but existing radioisotopic uptake assays only measure its passage through the latter bilayer, as the accumulation of the radionuclide in the cytoplasm. We devised a methodology that exclusively observes OM transport and used it to study the uptake of ferric enterobactin (FeEnt) by Escherichia coli FepA. This technique, called postuptake binding . . .
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