Mitochondrial genome engineering: the revolution may not be crispr-ized

HIGHLIGHTS

  • who: Payam A. Gammage from the Mitochondrial Biology Unit, University Cambridge, Cambridge, UK have published the paper: Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized, in the Journal: (JOURNAL)
  • what: It is helpful to underscore the often-contradictory findings reported in the studies discussed above by reference to valuable data from a comprehensive, quantitative analysis of the human mitochondrial transcriptome .
  • how: A mitochondrially targeted form of Cas9 demonstrated the same specific depletion of a single locus of mtDNA based on gRNA recognition and cleavage although no mtDNA sequencing data were presented . . .

     

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