HIGHLIGHTS
- who: Remy Villette from the Université University of have published the article: Refinement of 16S rRNA gene analysis for low biomass biospecimens, in the Journal: Scientific Reports Scientific Reports
- what: The authors demonstrate that 16S rRNA amplicon sequencing as a means to describe the phylogenetic composition of a microbiota sample is affected by_(1) microbial biomass, DNA extraction (mechanical cell lysing and DNA isolation) and_(3) PCR amplification. The authors show that microbial biomass is the most critical factor and only silica columns allow DNA isolation from the lowest microbial biomass samples ( and amp;lt . . .
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