Peroxisomal atp-binding cassette transporters form mainly tetramers

HIGHLIGHTS

• who: from the cline overnight) or not were homogenized in the solubilization buffer: , mM Tris-HCl, pH, mM NaCl, % detergent (C , (30), Triton, or ␤-DDM), mM PMSF, and protease inhibitor mixtures (Roche Diagnostics). Immunoprecipitation assays (from , to, ␮g of precleared cell lysate) were carried out as described previously (24). Bound proteins, including IP and co-IP proteins, were analyzed by SDS-PAGE, followed by immunoblotting with anti-ABCD1, anti-ABCD2, and anti-GFP antibodies. For co-IP assays coupled to mass spectrometry, the peroxisome-enriched fractions were prepared as described from the cloneexpressing or not expressing ABCD2 . . .
  

   

  

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