Maintaining mrna integrity during decalcification of mineralized tissues

HIGHLIGHTS

• who: Daniele Belluoccio and collaborators from the Murdoch Childrens Research Institute, Parkville, Victoria, Australia, Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, Victoria have published the research: Maintaining mRNA Integrity during Decalcification of Mineralized Tissues, in the Journal: PLOS ONE | www.plosone.org of July/27,/2012
• what: The authors show that this procedure also conserves the histological morphology which allows in situ hybridization analysis of mRNA and immunohistochemical analysis of protein localization.
• how: To overcome this limitation the authors developed a novel decalcification approach that preserves the integrity of the . . .
  

   

  

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