Process evaluation of recombinant chitin deacetylase expression in e. coli rosetta plyss cells using statistical design of experiments

HIGHLIGHTS

  • who: Bacillus aryabhattai and colleagues from the Manipal Institute of Technology have published the research: Process Evaluation of Recombinant Chitin Deacetylase Expression in E. Coli Rosetta pLysS Cells using Statistical Design of Experiments, in the Journal: (JOURNAL)
  • what: The experiments were carried out in triplicated and the experimental total activity was compared with predicted total activity under optimized conditions.
  • how: In this study five factors induction temperature (A) agitation rate (B) induction time (C) glucose concentration (D) and lactose concentration (E) were considered .

SUMMARY

    Chitin, a structural β 1-4 . . .

     

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