Electroporation of mice zygotes with dual guide rna/cas9 complexes for simple and efficient cloning-free genome editing

HIGHLIGHTS

• who: Marie Teixeira from the Lyon, Université Lyon, CNRS UMR, Ecole Normale Supérieure de Lyon, allée d'Italie, Lyon, France have published the paper: Electroporation of mice zygotes with dual guide RNA/Cas9 complexes for simple and efficient cloning-free genome editing, in the Journal: (JOURNAL) of 11/Dec/2017
• what: In the present manuscript the authors show the clear advantage of combining electroporation with the cloning-free procedure using dual guide RNA/SpCas9 RNP complexes to perform genome editing in mice.

SUMMARY

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