HIGHLIGHTS
- who: Kishore K. Chiruvella from the DepartmentStockholm University have published the article: Biochemical Characterization of Kat1: a Domesticated hAT-Transposase that Induces DNA Hairpin Formation and MAT-Switching, in the Journal: Scientific Reports Scientific Reports
- how: These data showed that Kat1 cuts TIR-R in the GTATAC site in_vitro. The strand-transfer activity of a transposase can be tested in_vitro by mixing a transposase with the TE and a target DNA molecule. The 13 mutants were tested in the strand-transfer assay using a pre-cleaved transposon end substrate in which the terminal 3-OH . . .
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