3′ tth endonuclease cleavage polymerase chain reaction (3tec-pcr) technology for single-base-specific multiplex pathogen detection using a two-oligonucleotide system

HIGHLIGHTS

• who: Owen Higgins and Terry J. Smith from the Molecular Diagnostics Research Group, School of Natural Sciences, National University of Ireland, Galway have published the Article: 3' Tth Endonuclease Cleavage Polymerase Chain Reaction (3TEC-PCR) Technology for Single-Base-Specific Multiplex Pathogen Detection using a Two-Oligonucleotide System, in the Journal: (JOURNAL)
• what: The authors have demonstrated 3TEC-PCR real-time detection, analytical specificity and sensitivity, SNP differentiation, and multiplex detection using bacterial meningitis pathogens Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pneumoniae as model target organisms.
• how: This article introduces 30 Tth endonuclease . . .
  

   

  

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