HIGHLIGHTS
- who: Yangyi Yu from the followsDeparaffinized and rehydrated samples were pretreated with specified enzymes (hyaluronidase , U m, for aggrecan and collagen II, chondroitinase ABC and keratinase I (, mU) for aggrecan, pepsin (, kU) for collagen X) for one hour at , u00b0C and antigen retrieval (collagen X and aggrecan). Sections were blocked with, % Normal Goat Serum (NGS) and, % Bovine Serum Albumin (BSA) in Tris Buffered Saline (TBS) (PEG, collagen II, and collagen X) or, % BSA in PBS (aggrecan) and permeabilized with, % BSA and, .25% Triton-X , in TBS (collagen II and collagen X) or, % BSA and, .25% Triton-X . . .
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