A broad g protein-coupled receptor internalization assay that combines snap-tag labeling, diffusion-enhanced resonance energy transfer, and a highly emissive terbium cryptate

HIGHLIGHTS

• who: Angélique Levoye from the Institut Nationnal Recherche Agronomique, France University of Toronto, Canada have published the article: A Broad G Protein-Coupled Receptor Internalization Assay that Combines Snap-tag Labeling, Diffusion-enhanced Resonance Energy Transfer, and a Highly Emissive Terbium Cryptate, in the Journal: (JOURNAL)
• what: The authors used a time-resolved FRET-based receptor internalization assay to characterize the dynamics of vasopressin, opioïd, chemokine, and glutamate receptors internalization in real-time in response to different ligand concentrations.
• how: The authors analyzed mGluR5 internalization by performing kinetic and dose-response . . .
  

   

  

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