A cell-based fluorescence resonance energy transfer (fret) sensor reveals inter- and intragenogroup variations in norovirus protease activity and polyprotein cleavage*

HIGHLIGHTS

• who: Edward Emmott from the Edward Emmott1, Trevor RSweeney1, and Ian Goodfellow, From the Division of Virology, Department of Pathology, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge , QQ, United Kingdom Background: Noroviruses generate the viral replicase through polyprotein cleavage by a viral protease. Results: A cell-based FRET sensor reveals significant variations in substrate cleavage both within and between norovirus genogroups. Conclusions: Norovirus proteases from different genogroups vary in their substrate recognition. Significance: Understanding the cleavage preferences of viral proteases is critical for drug design and provides insights into the potential for generating human-murine norovirus . . .
  

   

  

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