HIGHLIGHTS
- who: Catherine Anscombe from the Samples were extracted using the Qiasymphony-DSP mini kit , (Qiagen, UK) with offboard lysis or manually using the Qiagen mini viral extraction kitSamples were then tested using the CDC , assay to confirm the Ct values before sequencing. ARTIC protocol , sequencing protocol was used until June, after which we switched to the , protocol. ARTIC version , primers were used for the tiling PCR until we switched to the University of Zambia (UNZA) primer set that provided better results for Delta VOC in August , (data not shown) [20]. Initially two primer pools were used, however . . .
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