A conserved aromatic residue in the autochaperone domain of the autotransporter hbp is critical for initiation of outer membrane translocation*

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• who: Zora Soprova from the (pEH3-Hbp) by replacing a fragment using the KpnI and EcoRI restriction sitesThis resulted in plasmids pEH HbpF A and pEH3-HbpW A. A similar strategy was used to create the mutants W A, F A, W K, W F, and W Y. Plasmid pEH3-Hbp was mutated using nested PCR, essentially as described previously (39). The primers used for mutagenesis are listed in Table, . Nucleotide sequences were confirmed by semi-automated DNA sequencing. Reagents, Enzymes, and Sera-Dithiobis(succinimidyl) propionate (DSP) was purchased from Pierce. Restriction enzymes, DNA ligase, proteinase K . . .
  

   

  

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