A crispr-cas9 screen identifies exo1 as a formaldehyde resistance gene

HIGHLIGHTS

• who: Yuandi Gao from the Cell lines, antibodies, and reagents , hTeRT (RPE-1) cells were a gift from DrGraham Dellaire (Dalhousie University) and maintained in Dulbecco`s modified Eagle`s medium (DMEM) supplemented with, % fetal bovine serum (FBS) (Hyclone-Thermo Fisher Scientific, Ottawa, Canada). , hTeRT cells stably expressing Cas, (RPE-1-Cas9) were obtained from Dr. Dan Durocher (The Lunenfeld-Tanenbaum Research Institute, Toronto) and maintained in DMEM supplemented with, % FBS. Mouse embryonic fibroblasts (MEFs, p53-/-) proficient for , (MEF WT) or deficient for , (MEF KO) were generously provided by Dr. Winfried Edelmann (Albert Einstein College of Medicine . . .
  

   

  

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