A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

HIGHLIGHTS

  • who: Karen Davey from the DepartmentUniversity of have published the paper: A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle, in the Journal: Scientific Reports Scientific Reports
  • what: Technically speaking, the authors demonstrate a high integrity and quality of the transcriptome profiles generated when using the Nadia. With standard settings the authors report a low doublet rate of ~ 1% using recommended bead and cell loading densities (Fig 2a, Supplementary Figure 2), and favorable RNA capture efficiencies for both single cell and single nuclei sequencing compared to other reports . . .

     

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