A high-throughput screening rt-qpcr assay for quantifying surrogate markers of immunity from pbmcs

HIGHLIGHTS

  • who: Daniel J. Browne from the National Institute of Allergy and United States University of Texas Medical Branch at have published the research work: A high-throughput screening RT-qPCR assay for quantifying surrogate markers of immunity from PBMCs, in the Journal: (JOURNAL)
  • what: The authors demonstrate a very high mRNA/protein correlation between the HTS -optimized RT-qPCR protocol and ELIspot. The authors demonstrate no statistically significant loss in the correlation between mRNA and protein quantification post assay Frontiers in Immunology frontiersin.org 10.3389/fimmu.2022.962220 supported by a NHMRC Principal Research . . .

     

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