A high-throughput visual screening method for p-hydroxybenzoate hydroxylase to increase phenolic compounds biosynthesis

HIGHLIGHTS

  • who: Zhenya Chen from the (UNIVERSITY) have published the research: A high-throughput visual screening method for p-hydroxybenzoate hydroxylase to increase phenolic compounds biosynthesis, in the Journal: (JOURNAL)
  • what: In GA and biosynthetic pathways the low activity of p-hydroxybenzoate hydroxylase (PobA) towards 3 4-dihydroxybenzoic (3 4-DHBA) limited the high-level of GA and Results: This work reported a high activity PobA mutant (Y385F/T294A/V349A PobA) towards 3 4-DHBA. The high activity PobA which was obtained in this study has great potential for the production of GA or pyrogallol derivatives . . .

     

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