HIGHLIGHTS
- who: Yuliang Liu and Yaoqin Lu and Ailong Huang and Quanxin Long from the University of KwaZulu-Natal, South Africa have published the article: A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System, in the Journal: (JOURNAL)
- how: Based on the rational design and TMAS strategy the authors screened a set of crRNAs with desirable fluoroquinolone Target Mutation-Anchored Screening Strategy Enables CRISPR-Cas13a to Have Single-Base Specificity Previously data showed that a target mutation at the 50 region (position 3-6) combined with synthetic . . .
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