A method for disrupting conidia of neurospora

HIGHLIGHTS

  • who: M. Kapoor and D. Bray from the Disruption was produced with a microprobe sonifier (Branson Instruments, Inc). The conidia were suspended in IO ml of water (, x , cells per ml) in o thick glass tube, which was immersed in ice water. The microprobe of the sonifier was placed in the solution to a depth of I l/, cm. The sonifier was tuned to intensity level , and the current to between , and , amperes. The conidiol suspension was sonified for , seconds. In order to determine the success of the disruption technique, a malachite green stain was used. The . . .

     

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