A multiplex real-time reverse transcription polymerase chain reaction assay for differentiation of classical and variant ii strains of avian infectious bronchitis virus

HIGHLIGHTS

• who: Sara M. Ameen from the Department of Virology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt have published the article: A multiplex real-time reverse transcription polymerase chain reaction assay for differentiation of classical and variant II strains of avian infectious bronchitis virus, in the Journal: (JOURNAL)
• how: In this work several panels of reference materials as well as clinical samples were employed for analytical specificity testing and validation of the assay. Two independent real-time RT-PCR assays were conducted to identify classical and variant II genotypes discovered in chickens and the results . . .
  

   

  

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