A new cellular model to follow friedreich ’ s ataxia development in a time-resolved way

HIGHLIGHTS

• who: from the The pcDNA5-cFXN-FLAG expression vector was created by amplifying the full-length FXN cDNA gene carried by the pTLXI plasmid [kindly donated by Dr Mark Pook (Brunel University, London), FXN RefSeq FXNNM_000144) using , Hot Start High-Fidelity DNA polymerase (NEB) with primers, ′-ATATTGGATCCGCCACCATGTGGACTCTCGGGCGC-3′ and, ′-ATATTCTCGAGGGCGGCAGCATCTTTTCCGGAATAGGCC-3′, digested with BamHI and XhoI (NEB) and cloned in a modified pcDNA5™/FRT/TO inducible expression vector that carries at the C terminus of the gene of interest a, × FLAG tagTo make the pFSVpur-LoxP-TC-, targeting construct, Hot Start High-Fidelity DNA polymerase (NEB) was . . .
  

   

  

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