A new generation of t7 rna polymerase-independent inducible expression plasmids for trypanosoma brucei

HIGHLIGHTS

  • who: Jack Sunter and colleagues from the Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom, Centre for Genetics and Genomics, University of Nottingham, Nottingham, United have published the article: A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei, in the Journal: (JOURNAL) of April/12,/2012

SUMMARY

    A system for tetracycline-inducible transgene_expression in Trypanosoma brucei that is independent of T7 RNA_polymerase (T7 RNAP) is described. The remaining plasmids, p3227 for transgene_expression and p3666 for double stranded RNA expression, use the tetracycline-inducible EP1 procyclin promoter for . . .

     

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