HIGHLIGHTS
- who: Zhuguo Liu from the Oocyte electrophysiology was carried out as described previously44, Rat nAChR subunits α2, α3, α4, α7, β, and β, were in p, vector, α, in pGEMHE vector, α, in pSGEM vector and human α, in pMXT. Xenopus laevis oocytes were injected with , ng of cRNA, and then kept at , °C and maintained in sterile , buffer (, mM NaCl, mM KCl, ., mM CaCl2, mM MgCl2, and , mM HEPES, at pH, .4) supplemented with , mg/l gentamycin and , μg/IU per milliliter penicillin/streptomycin for, days before recording. All methods and experimental protocols were approved and carried out in accordance with . . .
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