A novel action of follicle-stimulating hormone in the ovary promotes estradiol production without inducing excessive follicular growth before puberty

HIGHLIGHTS

• who: Charlotte M. François from the Cultures of postnatal ovariesOvaries from , dpn female mice were placed on cell culture inserts (Millicell #PICM01250, Millipore, Guyancourt, France) on the top of , μ​l of culture media containing RPMI without red phenol, fetuin (Sigma, #F2379), insulin (Sigma, #I0516), transferrin (Sigma, #T8158), sodium selenite (Sigma, #S5261) and BSA (Euromedex, Souffelweyersheim, France) in, well plates for , hours. Then, the culture medium was replaced with , μ​l of fresh medium containing purified ovine pituitary FSH (50, or , ng/ml) provided by Dr R. Counis (University Paris-Diderot, France). After , hours of gonadotropin treatment, ovaries . . .
  

   

  

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