A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes

HIGHLIGHTS

• who: Manabu Abe from the DepartmentNiigata University have published the research: A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes, in the Journal: Scientific Reports Scientific Reports
• what: This investigation analyzed the potential of AAV vectors to serve as donor DNA for HR without prior infection. This study shows that knock-in rodents with multikilobase sequences can be generated using a very simple method. The authors successfully introduced AAV vectors along with CRISPR/Cas9 components through in situ electroporation, resulting in a knock-in of roughly 3 kb . . .
  

   

  

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