A novel tetrodotoxin-sensitive, voltage-gated sodium channel expressed in rat and human dorsal root ganglia*

HIGHLIGHTS

• who: Lakshmi Sangameswaran from the cDNA Cloning-Two rat DRG cDNA libraries, one oligo(dT) and the other random hexamer-primed, were constructed and screened as described (4)The cDNA probe for screening the random-primed cDNA library was a cDNA fragment from domains I and II of an rBIIa clone (10). Overlapping cDNA clones, .6, .5, and, ., were assembled in the Xenopus oocyte expression vector, pBSTA (16), at the AvrII/BspEI sites. The overlapping cDNA clones, .6, .5, .1, and, .1, the resulting clones at various stages of assembly, and the final p, clone were sequenced . . .
  

   

  

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