HIGHLIGHTS
- who: Mingchao Mu from the A total of , human CRC tissue were obtained from patients who had undergone open surgery for cancer resection at the First Affiliated Hospital of Xi`an Jiaotong University (Xi`an, China) between Dec, and Jan., . Tissues were then formalin-fixed, embedded in paraffin wax and sectioned into , u03bcm thick sections. The sections were incubated with primary antibodies against GSDME (1:200, ab215191, Abcam), (1:800, , AP, Proteintech) overnight at, u02daC, followed by HRP-conjugated secondary goat antibody (ZSGB-Bio). The staining intensity was divided into scores, , and , representing none, weak, moderate, and . . .
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