A peptide core motif for binding to heterotrimeric g protein α subunits*

HIGHLIGHTS

• who: William W. Ja from the EXPERIMENTAL PROCEDURES Materials-Human cDNA clones encoding various G proteins were obtained from the UMR cDNA Resource Center (wwwcdna.org) in the pcDNA3.1⫹ vector (Invitrogen). The G␣ subunits used were i1, i2, i3, oA, q, s (short form), and, . All in vitro translated G␤ and G␥ subunits refer to G␤, and, terminal hemagglutinin (HA)-tagged G␥2, respectively. Rat G␣i1, bovine short form G␣s and their chimeric constructs were generously provided by Prof. N. Artemyev (University of Iowa). DNA oligonucleotides were synthesized by Integrated DNA Technologies, Inc. (Coralville . . .
  

   

  

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