A phage display-based method for determination of relative affinities of mutants

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  • who: Stefaan Rossenu from the (UNIVERSITY) have published the article: A Phage Display-based Method for Determination of Relative Affinities of Mutants, in the Journal: (JOURNAL) of August/14,/2002
  • what: The authors propose phage display combined with enzymelinked immunosorbent assay as a tool for the systematic analysis of protein-protein interactions by investigating the binding behavior of variants to a partner protein. Via enzyme-linked immunosorbent assay the authors determine both the amount of fusion_protein presented at the phage surface and the amount of complex formed, the ratio of which is proportional to the . . .

     

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