A point mutation in escherichia coli dna helicase ii renders the enzyme nonfunctional in two dna repair pathways

HIGHLIGHTS

• who: Brosh Robert M. Jr. from the Bacterial Strains-Ecoli strain BL21(DE3) (l, ompT r, mB2) was obtained from Novagen, Inc. , (, lacZ dinD1::MudI (Aprlac)) was obtained from P. Model (Rockefeller University). BL21(DE3)DuvrD and JH DuvrD were previously constructed in this laboratory (30). DNA and Nucleotides-pBluescript (Stratagene) (pBS) was grown in E. coli , (, D(gpt-proA), leu, sup, ara, gal, lac, D(mrcC-mrr) rpsL20(Strr) xyl, mtl, recA13) and purified by alkalinesodium dodecyl sulfate (SDS) lysis followed by banding twice on CsCl2ethidium bromide gradients. Nicked pBS was prepared by digesting supercoiled DNA . . .
  

   

  

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