HIGHLIGHTS
- who: Emily K. Matozel and colleagues from the Editor: RVolkert, University of have published the article: A programmable DNA roadblock system using dCas9 and multivalent target sites, in the Journal: PLOS ONE of 16/02/2022
- what: The authors provide full protocols and sequences and demonstrate how to use the internal restriction sites to verify complete binding of the roadblock. The authors show a method for creating a programmable dCas9 roadblock intended for use in interfering with translocation by DBPs in_vitro. The authors provide complete protocols and sequences, as well as sample validation and experimental . . .
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