A rapid multiplex real-time pcr detection of toxigenic clostridioides difficile directly from fecal samples

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  • who: Xiao-xi Jia from the to the surveillance of CDI in China have published the article: A rapid multiplex real-time PCR detection of toxigenic Clostridioides difficile directly from fecal samples, in the Journal: (JOURNAL)
  • how: Were compared with established PCR qPCR and ELISA methods. To assess the repeatability of this method different concentrations (high medium and low copies) of three standard plasmids harboring tcdA tcdB and cdtB genes were tested in triplicate every other day. The results were compared with those of the gold standard (TC) method using tpi gene as internal control . . .

     

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