HIGHLIGHTS
SUMMARY
One avenue to enhance treatment efficacies and options is to learn how the disease agent Mycobacterium tuberculosis regulates gene_expression in response to stress. The main research question the authors are addressing is "how do mRNA metabolism and posttranscriptional regulation of gene_expression contribute to stress tolerance in mycobacteria?" Generally, the first 3 weeks were devoted to DNA manipulation and strain construction, followed by 3 weeks of analysis of gene_expression or growth phenotypes. Aware of cost, the authors chose to ligate a 50 nt singlestranded DNA adapter to 5′ monophosphorylated transcripts using T4 DNA_ligase (high concentration . . .
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