HIGHLIGHTS
- who: Ana Ruiz-Saenz from the Generation of GNAS lenti-CRISPR knockout cell linesGNAS lenti-CRISPR knockout cancer cell lines were generated by targeting exon , of the human GNAS locus with the CRISPR-Cas, system as previously described54, . The forward and reverse sgRNA-targeting sequences used were, u2032-CACCGCTACAACATGGTCATCCGGG-3u2032 and, u2032-AAACCCCGGATGACCATGTTGTAGC-3u2032, respectively. Guide sequences were provided by A. Inoue (Tohoku University). Oligonucleotides for the sgRNAs were phosphorylated and annealed for insertion into BsmBI-digested lenti-CRISPR v, backbone (Addgene, ). Lentiviruses were produced by transfecting HEK , cells with envelope, packaging and guide DNAs at a . . .
If you want to have access to all the content you need to log in!
Thanks :)
If you don't have an account, you can create one here.